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Altered dihydrofolate reductase in fol regulatory mutants of Escherichia coli K12

✍ Scribed by Sheldon, Robert

Publisher: Springer
Year: 1977
Tongue: English
Weight: 414 KB
Volume: 151
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00338697

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✦ Synopsis

Three spontaneous fol regulatory mutants contain dihydrofolate reductase molecules which differ in physical properties from enzymes of their parent strains. The enzymes were purified over 100-fold by affinity chromatography and were shown to differ in vitro in thermolability and in affinity for trimethoprim, a competitive inhibitor of the enzyme. These results indicate that some fol regulatroy mutations occur in the structural gene for dihydrofolate reductase.

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