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Regulatory mutants of dihydrofolate reductase in Escherichia coli K12

✍ Scribed by Sheldon, Robert ;Brenner, Sydney

Publisher: Springer
Year: 1976
Tongue: English
Weight: 668 KB
Volume: 147
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00337941

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✦ Synopsis

Trimethoprim inhibits dihydrofolate reductase. Mutations conferring trimethorpim-resistance on E coli K12 result in either an altered reductase with decreased affinity for the drug, or in 2-30 fold higher levels of the enzyme. Studies of the latter class of mutants indicate that dihydrofolate reductase is regulatdd by a diffusible molecule, and is probably under negative control. The regulatrory mutants, some of which are temperature-sensitive, act cis.

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