Allelic loss on the short arm of chromosome 11 in non-small-cell lung cancer

✍ Scribed by Christian U. Ludwig; Gaby Raefle; Peter Dalquen; Peter Stulz; Rolf Stahel; Jean-Paul Obrecht

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: French
• Weight: 505 KB
• Volume: 49
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910490506

✦ Synopsis

Forty-eight samples of primary non-small-cell lung cancer (NSCLC) and normal tissue from the same patients were analyzed for allelic deletions on chromosome I I p. Five polymorphic loci were assessed to determine the incidence of I Ip sequence deletions and to define hot-spots of deletions. Information was obtained from all patients in at least one locus. Our data show that the deletions observed were not randomly scattered over the short arm of chromosome I I. Rather, 2 hot-spots of deletions were observed: one in the area of the genes for catalase and p-FSH corresponding to band I I p 13, the other close to the IGF-ll locus corresponding to band I I p 15. A high incidence of loss of heterozygosity (LOH) was found with the probe for catalase (2 I /29), a locus flanking the centromeric region of the Wilms' tumor locus. Most of the samples exhibiting LOH of one or more of the alleles analyzed remained heterozygous for at least one other chromosome I Ip allele. Furthermore, duplication of the intensity of the remaining allele was rarely observed. Our results indicate that LOH on the short arm of chromosome I I is a common event in NSCLC and that the chromosomal region containing the Wilms' tumor locus is most commonly involved.