A methodology which combines tryptic digestions, 1,4-dithiothreitol reduction, and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS) allows verification of the location of the disulfide bridges in alpha-dendrotoxin (C7-C57, C16-C40 and C32-C53). Copyright 2000 John Wiley &
Alkaline cleavage of covalent bonds in chicken insulin and bovine α-lactalbumin analyzed by matrix-assisted laser desorption/ionization- mass spectrometry
✍ Scribed by Takashi Manabe; Ya Jin
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 480 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A number of potential matrix candidates were investigated with regard to the importance of the pH in the matrixassisted laser desorption/ionization mass spectrometry (MALDI/MS) analysis of non-covalently bound protein complexes. The matrices examined were 2,5-dihydroxybenzoic acid (DHB), 4-hydroxy-a
Matrix-assisted laser desorptionlionization (MALDI) has been employed for the determination of molecular weights of a-and &globins obtained from blood samples of healthy and diabetic subjects. Glycated species, originating from the reaction of glucose with the proteins, are easily evident. The MALDI
Ewe milk and ewe cheese samples were analysed by matrix-assisted laser desorption/ionization mass spectrometry and their protein profiles were compared with those obtained from bovine milk and bovine cheeses. Various mixtures of bovine and ewe cheeses in different weight ratios were analysed, leadin