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AHhaI/BstUI polymorphism in a novel gene at human chromosome 11p15.5

✍ Scribed by Kiyonori Miura; Hideaki Masuzaki; Tadayuki Ishimaru; Norio Niikawa; Y. Jinno

Publisher: Nature Publishing Group
Year: 1998
Tongue: English
Weight: 66 KB
Volume: 43
Category: Article
ISSN: 1435-232X
DOI: 10.1007/s100380050092

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## Detection conditions: Forward primer: 5´-ATT ACG TCC CAG CGT CTG AG-3Ŕ everse primer: 5´-ATA AAT CAG CTT GCT TAG TGC C-33 5 cycles; denaturation at 94°C (20 s), annealing at 60°C (20 s), extension at 72°C (20 s), final extension 72°C (10 min) 10% PAA gels (49:1, acrylamide:bisacrylamide), 0.5XTB