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Novel intronic polymorphism (+1675G/A) in the human angiotensin II subtype 2 receptor gene

✍ Scribed by Jeanette Erdmann; Marco Guse; Heike Kallisch; Eckart Fleck; Vera Regitz-Zagrosek

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 12 KB
Volume: 15
Category: Article
ISSN: 1059-7794
DOI: 10.1002/(sici)1098-1004(200005)15:5<487::aid-humu29>3.0.co;2-d

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✦ Synopsis

Detection conditions:

Forward primer: 5´-ATT ACG TCC CAG CGT CTG AG-3Ŕ everse primer: 5´-ATA AAT CAG CTT GCT TAG TGC C-33 5 cycles; denaturation at 94°C (20 s), annealing at 60°C (20 s), extension at 72°C (20 s), final extension 72°C (10 min) 10% PAA gels (49:1, acrylamide:bisacrylamide), 0.5XTBE / 18hrs / 4°C / 70 Volts. The bands are visualized by silver-staining.

Diagnosis method developed:

Since this polymorphism does not alter any known enzyme restriction site and no ACRS approach with subsequent restriction analysis could be established, the genotyping was based on SSCP analysis.

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