## Detection conditions: Forward primer: 5ยด-ATT ACG TCC CAG CGT CTG AG-3ล everse primer: 5ยด-ATA AAT CAG CTT GCT TAG TGC C-33 5 cycles; denaturation at 94ยฐC (20 s), annealing at 60ยฐC (20 s), extension at 72ยฐC (20 s), final extension 72ยฐC (10 min) 10% PAA gels (49:1, acrylamide:bisacrylamide), 0.5XTB
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A novel intronic polymorphism (intron 2 +130 (CT)n) in the human homeobox gene HOXB3
โ Scribed by Susan A. Copeland-Yates; Ron C. Michaelis
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 17 KB
- Volume
- 17
- Category
- Article
- ISSN
- 1059-7794
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The retinoblastoma gene (RB) encodes a tumor suppressor that is inactivated in a number of different types of cancer. We searched for gross alterations of this gene in tumors of the central nervous system by using Southern blot hybridization. A common alteration was found in several tumors and was m