Affinity purification of glutamate synthase from Escherichia coli
โ Scribed by C.N.G. Schmidt; L. Jervis
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 230 KB
- Volume
- 104
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
A simple, sensitive fluorometric assay for L-glutamine is described. r-Glutamine is quantified by measuring the amount of NADPH oxidized in the presence of a glutamine-containing sample (2-20 nmol), 5 mM a-ketoglutarate, 1 mM EDTA. 63 PM NADPH. and purified Escherichia co/i glutamate synthase (EC 2.
Highly active salt-washed ribosomes from Escherichia coli are isolated by gel filtration on Sephacryl S-200 in a buffer containing 1 M ammonium chloride and putrescine, spermidine, calcium, and magnesium ions. Up to several hundred grams of cells can be processed in less than 24 h. The ribosome solu