✦ LIBER ✦
Quantification of l-glutamine using Escherichia coli glutamate synthase: A sensitive fluorometric assay
✍ Scribed by Richard E. Miller
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 568 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
A simple, sensitive fluorometric assay for L-glutamine is described. r-Glutamine is quantified by measuring the amount of NADPH oxidized in the presence of a glutamine-containing sample (2-20 nmol), 5 mM a-ketoglutarate, 1 mM EDTA. 63 PM NADPH. and purified Escherichia co/i glutamate synthase (EC 2.6.1.53). High concentrations of L-glutamate and ammonia do not interfere. The method has been applied to quantification of L-glutamine in serum.