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Affinity chromatography: Specific binding of hemoglobin on agarose linked haptoglobin

✍ Scribed by Michel Klein; Constantin Mihaesco

Book ID
118854203
Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
269 KB
Volume
52
Category
Article
ISSN
0006-291X

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Affinity chromatography of a sequence-sp
Affinity chromatography of a sequence-specific DNA binding protein using teflon-linked oligonucleotides
✍ Craig H. Duncan; Sharon L. Cavalier πŸ“‚ Article πŸ“… 1988 πŸ› Elsevier Science 🌐 English βš– 594 KB

An affinity matrix was constructed by synthesis of a DNA oligonucleotide on a Teflon fiber support followed by deblocking and hybridization of the complementary strand. It was used to purify a sequence-specific binding protein at least 100-fold to near homogeneity. This matrix is easily fabricated o