The lectin jacalin immobilized on agarose was found to bind a variety of glycoproteins known to contain typical O-linked oligosaccharides, including human IgA, C1 inhibitor, chorionic gonadotropin, plasminogen, bovine protein Z, bovine coagulation factor X, and fetuin. These proteins were eluted fro
β¦ LIBER β¦
Isolation of glycopeptides containing O-linked oligosaccharides by lectin affinity chromatography on jacalin-agarose
β Scribed by Glen L. Hortin
- Book ID
- 107714852
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 623 KB
- Volume
- 191
- Category
- Article
- ISSN
- 0003-2697
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The seeds of Griffonia simplicifolia contain a family of five isolectins (GS-I) (L. A. Murphy and I. J. Goldstein (1977) J. Biol. Chem. 252, 4739-4742) that bind with high affinity to glycoconjugates containing terminal nonreducing alpha-linked galactose residues. Here, we report that GS-I itself is