Separation of oligosaccharides containing terminal α-linked galactose residues by affinity chromatography on Griffonia simplicifolia I bound to concanavalin A-Sepharose
✍ Scribed by Wei-Chun Wang; Gary F. Clark; David F. Smith; Richard D. Cummings
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 567 KB
- Volume
- 175
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
The seeds of Griffonia simplicifolia contain a family of five isolectins (GS-I) (L. A. Murphy and I. J. Goldstein (1977) J. Biol. Chem. 252, 4739-4742) that bind with high affinity to glycoconjugates containing terminal nonreducing alpha-linked galactose residues. Here, we report that GS-I itself is bound via its high mannose-type, Asn-linked sugar chains to immobilized concanavalin A (Con A-Sepharose). The GS-I in the GS-I-Con A-Sepharose complex retains its ability to bind glycoconjugates containing terminal alpha-linked galactose residues. This convenient method to immobilize GS-I is rapid and quantitative. We have exploited this affinity system to separate oligosaccharides based on their number of terminal alpha-linked D-galactose residues.