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Aerobic ferrisiderophore reductase assay and activity stain for native polyacrylamide gels

โœ Scribed by Mark D. Moody; Harry A. Dailey

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
432 KB
Volume
134
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

The reduction of ferric iron from microbial iron-binding compounds (siderophores) releases the iron from the siderophore so that it may be utilized by the microorganism. A method to detect aerobic ferrisiderophore reductase activity using ferrozine as a ferrous iron trap is shown to be applicable to cytoplasmic fractions from Rhodopseudomonas sphaeroides and four other different species of bacteria. The ferrisiderophore reductase uses reduced nicotinamide cofactors as reducing agents, and activity is stimulated by flavins. This assay has been adapted as a staining method to locate ferrisiderophore reductase activity in native polyacrylamide gels.

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