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Method for Activity Staining of Peroxidase and β-1,3-Glucanase Isozymes Polyacrylamide Electrophoresis Gels

✍ Scribed by M. Shimoni


Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
299 KB
Volume
220
Category
Article
ISSN
0003-2697

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A Method for Activity Staining after Nat
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We describe a method for the detection of isoforms of several glycolytic enzymes by activity staining after native PAGE. The staining is based on coupled enzyme assays carried out on the gel after electrophoresis and is linked to the disappearance of NADH, which is visualized by fluorescence. This m