Advances in DNA electrophoresis in polymer solutions
β Scribed by Dietmar Tietz; Akram Aldroubi; Helena Pulyaeva; Tadeusz Guszczynski; Mark M. Garner; Dr. Andreas Chrambach
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 359 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0173-0835
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## Abstract Capillary electrophoresis (CE) in entangled polymer solutions was studied using pBR322 DNA Hae III digest as a model sample. The influences of polymer nature, monomer chain length, and polymer concentration were investigated. The use of a novel polymer, galactomannan, as the entangled m
Studies of electrophoretic separations of DNA restriction fragments by capillary electrophoresis in solutions of (hydroxyethyl) cellulose (HEC) were performed. Rheological studies were used to confirm that the entanglement threshold (a\* ) for the HEC solutions used is approximately 0.004 g/mL, in g