Advanced Optical Flow Cytometry: Methods and Disease Diagnoses

✍ Scribed by Valery V. Tuchin

• Publisher: Wiley-VCH
• Year: 2011
• Tongue: English
• Leaves: 740
• Edition: 1
• Category: Library

✦ Synopsis

A detailed look at the latest research in non-invasive in vivo cytometry and its applications, with particular emphasis on novel biophotonic methods, disease diagnosis, and monitoring of disease treatment at single cell level in stationary and flow conditions.This book thus covers the spectrum ranging from fundamental interactions between light, cells, vascular tissue, and cell labeling particles, to strategies and opportunities for preclinical and clinical research. General topics include light scattering by cells, fast video microscopy, polarization, laser-scanning, fluorescence, Raman, multi-photon, photothermal, and photoacoustic methods for cellular diagnostics and monitoring of disease treatment in living organisms. Also presented are discussions of advanced methods and techniques of classical flow cytometry.

✦ Table of Contents

CoverPage......Page 1
FrontMatter......Page 4
Contents......Page 6
Preface......Page 22
Contributors......Page 32
1 Perspectives in Cytometry......Page 40
2 Novel Concepts and Requirements in Cytometry......Page 64
3 Optical Imaging of Cells with Gold Nanoparticle Clusters asLight Scattering Contrast Agents......Page 74
4 Optics of White Blood Cells: Optical Models, Simulations, and Experiments......Page 102
5 Optical Properties of Flowing Blood Cells......Page 134
6 Laser Diffraction by the Erythrocytes and DeformabilityMeasurements......Page 172
7 Characterization of Red Blood Cells’ Rheological andPhysiological State Using Optical Flicker Spectroscopy......Page 194
8 Digital Holographic Microscopy for Quantitative Live CellImaging and Cytometry......Page 250
9 Comparison of Immunophenotyping and Rare Cell Detectionby Slide-Based Imaging Cytometry and Flow Cytometry......Page 278
10 Microfluidic Flow Cytometry......Page 312
11 Label-Free Cell Classification with Diffraction Imaging FlowCytometer......Page 350
12 An Integrative Approach for Immune Monitoring of HumanHealth and Disease by Advanced Flow Cytometry Methods......Page 372
13 Optical Tweezers and Cytometry......Page 402
14 In vivo Image Flow Cytometry......Page 426
15 Instrumentation for In vivo Flow Cytometry......Page 472
16 Advances in Fluorescence-Based In vivo Flow Cytometryfor Cancer Applications......Page 502
17 In vivo Photothermal and Photoacoustic Flow Cytometry......Page 540
18 Optical Instrumentation for the Measurement of BloodPerfusion, Concentration, and Oxygenation......Page 612
19 Blood Flow Cytometry and Cell Aggregation Study with LaserSpeckle......Page 644
20 Modifications of Optical Properties of Blood duringPhotodynamic Reactions In vitro and In vivo......Page 666
Index......Page 738