Imaging Flow Cytometry: Methods and Protocols

✍ Scribed by Natasha S. Barteneva, Ivan A. Vorobjev

• Publisher: Humana Press
• Year: 2015
• Tongue: English
• Leaves: 311
• Series: Methods in Molecular Biology 1389
• Category: Library

✦ Synopsis

This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.

✦ Table of Contents

Front Matter....Pages i-xiii
Front Matter....Pages 1-1
Front Matter....Pages 3-11
Front Matter....Pages 13-21
Front Matter....Pages 23-45
Front Matter....Pages 47-67
Back Matter....Pages 69-69
....Pages 71-95

✦ Subjects

Imaging / Radiology