Flow and Image Cytometry

✍ Scribed by Alemseged Truneh, Manjula Reddy, Patricia Ryan, Sally D. Lyn, Ilona Kariv (auth.), Alain Jacquamin-Sablon (eds.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 1996
• Tongue: English
• Leaves: 245
• Series: NATO ASI Series 95
• Edition: 1
• Category: Library

✦ Synopsis

Image analysis and flow cytometry are complementary techniques which provide new insights into essential aspects of cell biology. This book presents an up-to-date overview of current ideas and methods in these domains. It consists of three parts. Part I, "Membrane Dynamics and Function", deals with adhesion molecules, membrane pump dynamics, membrane fusion and endocytosis, membrane potential, T-cell homing and cytoskeleton, etc. The second part focuses on problems of cell proliferation, chromosome analysis and sorting, apoptosis, imaging cytometry of fixed and living cells, image analysis for gene mapping from flow-sorted chromosomes, chromatin organization, and liposome-mediated delivery of DNA binding drugs. The third part covers data management systems, cell sorting techniques and microscopy.

✦ Table of Contents

Front Matter....Pages I-VIII
Front Matter....Pages 1-1
Analysis of CD28 Interactions with Its Cognate Counter-Receptors CD80 and CD86....Pages 3-19
Membrane Transport Dynamics....Pages 21-46
Endogenous lectins in circulating cells and their glycosylated ligands: their role in T cell homing....Pages 47-51
Use of Different Cytometric Techniques to Study the Cytotoxic Interaction between Human Natural Killer Cells and k562 Target cells....Pages 53-68
In vitro Reconstitution of Early Endosome Membrane Dynamics....Pages 69-80
Change in Membrane Fluidity after pinocytosis of a new Cytokine IK on Resting and Activated T cells....Pages 81-87
Front Matter....Pages 89-89
Cell Cycle and Cell Proliferation Markers....Pages 91-101
Advances in Flow Cytogenetics: Progress in the Development of a High Speed Optical Chromosome Sorter Based on Photoinduced Adduct Formation Between Psoralens and Chromosomal DNA....Pages 103-114
Analysis of Cell Death by Flow Cytometry....Pages 115-130
Molecular Cytogenetics: Uses of flow sorted chromosomes, fluorescence in situ hybridisation (FISH) and digital microscopy for the analysis of genomes....Pages 131-141
DNA Topoisomerases as Drug Targets and Cell Cycle Checkpoint Effector Molecules....Pages 143-153
Hemopoietic Cell Differentiation and Death By Retinoids....Pages 155-165
Front Matter....Pages 167-167
Myc in the Control of Proliferation and Apoptosis....Pages 169-178
Flow Cytometry: Analyses for All Sizes....Pages 179-190
Flow Cytometric Immunophenotyping Using Cluster Analysis And Cluster Editing....Pages 191-212
Introduction to High-Speed Flow Sorting....Pages 213-227
Applications of Near-field Microscopes to Cell Biology....Pages 229-236
Back Matter....Pages 237-248

✦ Subjects

Cell Biology; Biochemistry, general; Immunology