Adenovirus-Mediated Regulable Target Gene Expression in vivo
β Scribed by Mark M. Burcin, Gudrun Schiedner, Stefan Kochanek, Sophia Y. Tsai and Bert W. O'Malley
- Book ID
- 123641738
- Publisher
- National Academy of Sciences
- Year
- 1999
- Tongue
- English
- Weight
- 997 KB
- Volume
- 96
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/46815
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We used recombinant adenoviruses as a means of expressing exogenous genes in olfactory neurons in vivo. A replication incompetent adenovirus (type 5, Ad5) carrying the reporter gene lacZ, which codes for the enzyme 8-galactosidase (,&Gal), was applied in solution to the olfactory epithelia of rats.
## Abstract We report here a novel approach to direct gene expression in the mouse somite based on the combined application of adenovirusβmediated gene delivery and whole embryo ex vivo culture. As proof of principle, we show functional analysis of somites microinjected with an engineered virus exp