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Targeting gene expression in the mouse somite: Adenovirus-mediated gene delivery and whole embryo culture

✍ Scribed by Alice E. Chen; Chen-Ming Fan

Publisher: John Wiley and Sons
Year: 2005
Tongue: English
Weight: 355 KB
Volume: 42
Category: Article
ISSN: 1526-954X
DOI: 10.1002/gene.20126

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✦ Synopsis

Abstract

We report here a novel approach to direct gene expression in the mouse somite based on the combined application of adenovirus‐mediated gene delivery and whole embryo ex vivo culture. As proof of principle, we show functional analysis of somites microinjected with an engineered virus expressing an activated form of Smoothened, the signaling receptor for Sonic Hedgehog (SHH). As adenovirus can infect many embryonic tissues in the mouse, this method may provide an effective alternative to conventional transgenesis for targeted spatial and temporal gene expression. genesis 42:71–76, 2005. © 2005 Wiley‐Liss, Inc.

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