Activity-based fluorescent probes that target phosphatases

✍ Scribed by Qing Zhu; Xuan Huang; Grace Y.J. Chen; Shao Q. Yao

• Book ID: 104253235
• Publisher: Elsevier Science
• Year: 2003
• Tongue: French
• Weight: 327 KB
• Volume: 44
• Category: Article
• ISSN: 0040-4039
• DOI: 10.1016/s0040-4039(03)00348-4

✦ Synopsis

We have successfully designed and synthesized two fluorescently-labeled, activity-based probes, Probe 1 and Probe 2, which were shown to label protein tyrosine phosphatases specifically, as well as other types of phosphatases. The probes were not reactive towards the other non-phosphatase enzymes tested. These probes may find potential applications in large-scale proteomic experiments whereby subclasses of proteins may be selectively identified.