Activation of protease by sol-gel entrapment into organically modified hybrid silicates
β Scribed by Shin-ya Furukawa; Tsutomu Ono; Hiroyuki Ijima; Koei Kawakami
- Book ID
- 110325106
- Publisher
- Springer Netherlands
- Year
- 2002
- Tongue
- English
- Weight
- 76 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0141-5492
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Candida cylindracea lipase was entrapped in methyl-substituted organic silicates formed on kieselguhr such as Celite 545 and Hyflo Super-Cel. Various preparation conditions were optimized to obtain a more thermostable immobilized lipase. The hybrid gel-entrapped lipase derived from an equimolar mixt
Silica glass-entrapped lipase was prepared by the sol-gel method using tetramethoxysilane, and its esterification activity in n-hexane was examined for isoamylbutyrate formation. The hydrogel preparation containing a large amount of water exhibited enough activity. Although the activity of xerogel-e