Enhancement of thermostability of lipase by the sol-gel entrapment into methyl-substituted organic silicates formed on diatomaceous earth

✍ Scribed by Koei Kawakami

• Book ID: 104648691
• Publisher: Springer-Verlag
• Year: 1996
• Tongue: English
• Weight: 342 KB
• Volume: 10
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00159512

✦ Synopsis

Candida cylindracea lipase was entrapped in methyl-substituted organic silicates formed on kieselguhr such as Celite 545 and Hyflo Super-Cel. Various preparation conditions were optimized to obtain a more thermostable immobilized lipase. The hybrid gel-entrapped lipase derived from an equimolar mixture of trimethylmethoxysilane and tetramethoxysilane, retained its full activity for ester synthesis up to the temperatures of 65°C.