Activation of oncogene c-Ha-ras in gastric cancer of chinese patients

✍ Scribed by Guoren Deng; Zheng Eh; Yi Xu; Youyong Lu

• Book ID: 104594207
• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 477 KB
• Volume: 10
• Category: Article
• ISSN: 8756-0437
• DOI: 10.1002/ssu.2980100204

✦ Synopsis

A transforming gene was cloned from a focus of rat fibroblast cell line Rat 1 transfected with DNA of a gastrocarcinoma cell line. The transforming gene is the activated form of oncogene c-Hams, and the lesion was identified as a single nucleotide substitution at codon 12 by sequencing. We further used polymerase chain reaction (PCR)-restriction analysis to detect the mutations at codon 12 of c-Ha-ras. By using this method, 11 cases out of 24 solid tumors and 3 cell lines of gastric cancer were shown to contain mutations, and there was a strong correlation between mutations and metastasis and survival of the patients. The role of the activated c-Ha-ras in tumorigenesis of stomach cancer was further proved through the assay in which the malignant phenotypes of the c-Ha-ras-transformed cells were partially inhibited by blocking the c-Ha-ras expression with antisense oligonucleotides or exogenous plasmid expressing antisense RNA.