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Activation of benzo(a)pyrene-7,8-dihydrodiol in rat uterus: Anin vitro study

✍ Scribed by Byczkowski, Janusz Z. ;Kulkarni, Arun P.

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
673 KB
Volume
5
Category
Article
ISSN
0887-2082

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✦ Synopsis

Peroxidatic metabolism of benzo( a ) pyrene-7,S-dihydrodiol by calcium containing extracts of rat uteri was investigated. Covalently bound and soluble metabolites of benzo( a)pyrene-7,S-dihydrodiol were quantitated by radiometry and high performance liquid chromatography, respectively. 1. Uterine extracts incubated with benzo( a)pyrene-7,s-dihydrodiol activated this proximate mutagen to protein binding metabolite(s). 2. Hydrogen peroxide increased the protein binding and yielded a substantial amount of benzo( a ) pyrene-trans-anti-tetrahydrotetrol, suggesting the peroxyl-type free-radical epoxidation process. 3. The results indicate that rat uterine peroxidase is able to catalyze free-radical activation of benzo ( a)pyrene4,8-dihydrodiol by epoxidation to its 9,10-dihydrodiolepoxide, a known ultimate mutagen and carcinogen.

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