Activation of amino acid diffusion by a volume increase in cultured kidney (MDCK) cells

✍ Scribed by G. Roy; C. Malo

• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 759 KB
• Volume: 130
• Category: Article
• ISSN: 0022-2631
• DOI: 10.1007/bf00233740

✦ Synopsis

When MDCK cells are cultured in MEM, they maintain a high concentration of three amino acids: glutamate (25 mM), taurine (19 mM) and glycine (9 mM). With incubation of the cells in hypotonic media, the contents of these amino acids measured by HPLC are reduced in different time courses: taurine decreases most rapidly, followed by glutamate and glycine. All these losses are Na+ independent. To determine the transport mechanism activated by the hypotonic media, increasing external concentrations reaching 60 mM for nine different amino acids in Na(+)-free media were tested separately. For the five neutral (zwitterionic) amino acids, taurine, glycine, alanine, phenylalanine and tryptophan, cell contents increased linearly with external concentrations in hypotonic media, whereas in isotonic media only a slight rise was observed. The two anionic amino acids, glutamate and aspartate, were also increased linearly with their external concentrations in hypotonic media, but the changes were lower than those found for neutral amino acids. The presence of a negative membrane potential was responsible for this behavior since, using a K+ hypotonic medium which clamps the potential to zero, the glutamate content was found to increase linearly with an amplitude similar to the one observed for neutral amino acid. When external concentrations of two cationic amino acids, arginine and lysine, were increased in hypotonic media, only a small change, similar to that in isotonic media, was observed. These results indicate that a diffusion process for neutral and anionic amino acids is activated by a volume increase and it is suggested that an anion channel is involved.