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Hormonal regulation of the system a amino acid transport adaptive response mechanism in a kidney epithelial cell line (MDCK)

✍ Scribed by Paula Boerner; Milton H. Saier Jr.

Publisher: John Wiley and Sons
Year: 1985
Tongue: English
Weight: 679 KB
Volume: 122
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041220222

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✦ Synopsis

When mamalian cells are starved for amino acids, the activity of the A amino acid transport system increases, a phenomenon called adaptive regulation. We have examined the effects of those factors which support Madin-Darby canine kidney (MDCK) cell growth in a defined medium on the derepression of System A activity. Of the five factors which supported MDCK cell growth, insulin was found to be an absolute requirement for derepression. In contrast, PGE, was a negative controlling factor for the transport system. Growth of MDCK cells in the absence of PGE, resulted in elevated System A activity

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