Action of some retinol derivatives and their provitamins on microsome-catalyzed formation of benzo[a]pyrene-DNA adduct

✍ Scribed by Shah, G. M. ;Goswami, U. C. ;Bhattacharya, R. K.

• Book ID: 102875647
• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 512 KB
• Volume: 7
• Category: Article
• ISSN: 0887-2082
• DOI: 10.1002/jbt.2570070307

✦ Synopsis

Several vitamin A compounds have been tested for their ability to suppress formation of DNA adduct by the carcinogen benzo[alpyrene (B[a]P) in an in vitro reaction catalyzed by rat liver microsomes. Retinol, retinal, 3-dehydroretinol and 3-hydroxyretinol were found to be effective inhibitors of adduct formation. Certain carotenoids that are precursors of these retinoids also displayed considerable inhibitory capacity. Carotenoids and the 3-substituted retinoids appeared to modulate the DNA adduct formation exclusively through their action on rnicrosomal enzymes, since an effective inhibition in each case was observed on the formation of B[a]P-7,8-diol, a proximate carcinogenic metabolite of B[a]P. Unsubstituted retinoids, on the other hand, had marginal effect on enzymes but were found effective in accelerating inactivation of B[a]P-7,8-diol-9,10-epoxide, the ultimate carcinogenic metabolite that binds to DNA.