Action of Nucleotide Phosphotransferase of Escherichia coli on Nicotinamide Riboside and Nicotinamide Mononucleotide
β Scribed by Elinor F. Brunngraber and Erwin Chargaff
- Book ID
- 123654823
- Publisher
- National Academy of Sciences
- Year
- 1977
- Tongue
- English
- Weight
- 573 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/67693
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The conditions of extraction of nicotinamide nucleotides from Escherichia coli were systematically investigated. The optimum pH for NAD+ and NADP+ appeared to be around 1.5 while that for NADH and NADPH was around 12.5. Seven minutes at 60Β°C was chosen for the extraction of all four nucleotides. Add
Regulatory roles of nicotinamide nucleotides and three key enzymes, p-ketothiolase (KT), NADPH-dependent acetoacetyl-CoA reductase (AAR), and citrate synthase (CS), on poly(3-hydroxybutyrate) (PHB) synthesis in recombinant Escherichia coli harboring a plasmid containing the Alcaligenes eutrophus pol