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Acid citrate dextrose (acd) formula a as a new anticoagulant in the measurement of in vitro platelet aggregation

✍ Scribed by Pasquale Pignatelli; Fabio M. Pulcinelli; Filippo Ciatti; Marzia Pesciotti; Silvia Sebastiani; Patrizia Ferroni; Pier Paolo Gazzaniga

Publisher: John Wiley and Sons
Year: 1995
Tongue: English
Weight: 302 KB
Volume: 9
Category: Article
ISSN: 0887-8013
DOI: 10.1002/jcla.1860090211

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✦ Synopsis

To evaluate whether the use of ACD Formula A may affect in vitro platelet function, blood samples were obtained from 21 healthy blood donors and anticoagulated in ACD (acid-citrate dextrose, NIH Formula A), Na citrate 3.8%, and K3EDTA. Platelet count, mean platelet volume, and in vitro platelet aggregation were evaluated on each sample. No significant difference was observed in platelet count and mean platelet volume among the different samples. Conversely, theACD treated platelets showed a higher reactivity to the agonists as demonstrated by a significant increase of the maximum percentages of aggregation induced by ADP, epinephrine, and collagen, as well as a significant decrease of secondary aggregation thresholds to ADP and epinephrine. In conclusion, it may be speculated that ACD Formula A is capable of better maintaining the intraplatelet signal transduction mechanisms during PRP preparation, thus improving the overall responsiveness of platelets.

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