Accurate and efficient method for quantification of urinary histamine by gas chromatography negative ion chemical ionization mass spectrometry

✍ Scribed by L.Jackson Roberts II; John A. Oates

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 486 KB
• Volume: 136
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(84)90333-6

✦ Synopsis

Because of the recognized inaccuracy and unreliability of currently available methods for the quantification of histamine in biological fluids, a method for quantification of urinary histamine by stable isotope dilution assay with negative ion chemical ionization mass spectrometry has been developed. Following the addition of [2H4]histamine to 1 ml of urine, histamine is extracted into butanol, back-extracted into HCl, derivatized to the pentafluorobenzyl derivative (CH,C&,),histamine, extracted into methylene chloride, and then quantified with negative ion chemical ionization mass spectrometry by selected ion monitoring of the ratio of ions m/z 430/434. Twenty samples can be assayed in 2 days. Precision of the assay is +2.7% and the accuracy is 97.6%. Lower limits of sensitivity are approximately 100-500 fg injected on-column. This assay provides a very sensitive, accurate, and efficient method for the quantification of histamine in human urine.