✦ LIBER ✦

Determination of Cortisol in Human Plasma by Gas Chromatography/Negative Ion Chemical Ionization/ Mass Spectrometry

✍ Scribed by F.F. Hsu; L.H. Wang; D.M. Bier

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 296 KB
Volume: 216
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1994.1058

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✦ Synopsis

We report an isotope dilution mass spectrometry method using capillary gas chromatography negative ion chemical ionization for the determination of cortisol in human plasma. Cortisol was derivatized with pentafluoropropionic anhydride at room temperature and measured by selected ion monitoring of the molecular ion. This method utilized (d_{4})-cortisol as an internal standard and required only a simple correction for the isotope contribution. A sensitivity as low as (0.02 \mathrm{pmol}(8 / n) (>10)) can be achieved easily. Therefore a plasma sample as small as (0.1 \mathrm{ml}) can be used for the accurate measurement of cortisol in humans. Cc 1994 Academic Press, Inc.

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