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Acceleration of DNA renaturation rates

โœ Scribed by James G. Wetmur

Publisher
Wiley (John Wiley & Sons)
Year
1975
Tongue
English
Weight
351 KB
Volume
14
Category
Article
ISSN
0006-3525

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โœฆ Synopsis

Abstract

The rate of renaturation of DNA may be increased by altering the effective solvent volume available for DNA in solution. Accelerations are demonstrated when neutral and anionic dextran polymers are used to exclude volume in DNA solutions. The logarithm of the DNA renaturation rate constant is shown to be proportional to the concentration of dextran polymer and to be proportional to the intrinsic viscosity of a series of dextran polymers of identical chemical composition. A theory is proposed to account for these results.

๐Ÿ“œ SIMILAR VOLUMES

Determination of the rate of renaturatio
Determination of the rate of renaturation of modified DNA by fluorescence depolarization
โœ Stephen J. Miller; James G. Wetmur ๐Ÿ“‚ Article ๐Ÿ“… 1974 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 326 KB

## Abstract Fluorescence depolarization was used to measure the rate of renaturation of T2 DNA, which had been modified by chloroacetaldehyde. Rates were measured on DNA samples with 5โ€“15% of the base pairs modified and were found to agree with rates determined by DNA absorbance kinetics at 260 nm.

Renaturation of condensed DNA studied th
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## Rapid Communications of charge 3/ or greater [such as the polyamines spermidine (3/) and spermine (4/), or the inorganic cation cobalthexamine (3/)]. DNA condensation can lead to the collapse (a coil to globule transition) of long chains at very low concentrations. Under most experimental condit

Physical properties of endonuclease S1 d
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โœ Stephen J. Miller; James G. Wetmur ๐Ÿ“‚ Article ๐Ÿ“… 1975 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 477 KB

## Abstract The growth of the weightโ€average doubleโ€strand length of renaturing sonicated DNA was investigated as a function of the degree of renaturation. Endonuclease S1 was used to digest singleโ€strand ends and gaps. The ratio of the average renatured length to the initial length was found to be

Renaturation of DNA in ethanolโ€“methanol
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โœ Bengt Nordรฉn; Stefan Seth; Folke Tjerneld ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 160 KB

The conformation of DNA in alcohol solution has been studied recently using the technique of circular dichroism.14 It is accepted that DNA adopts its C-form in methanol in the presence of 5% SSC buffer.\* However, ideas about the predominant conformation in a mixed solvent of methanol and ethanol (1