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Absorption spectra of Δ5-3β-hydroxy steroids in two reagents

✍ Scribed by Alfred M. Bongiovanni

Publisher
Elsevier Science
Year
1965
Tongue
English
Weight
277 KB
Volume
13
Category
Article
ISSN
0003-2697

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✦ Synopsis

In the course of studies of A5-3P-hydroxy steroids in human urine, the absorption spectra of these compounds in two sulfuric acid reagents proved of value in arriving at their identification (1). Wilson (2) has previously reported spectra of 11 steroids in sulfuric acid reagents. Smith et al. ( 3) have compiled spectra for a large number of steroids in sulfuric acid. This report extends these observations to 10 additional compounds and includes 5 previously described. These methods arc helpful for the partial characterization of steroids in small quantity and as noted below one pair of isomers may be distinguished by these techniques.

MATERIALS AND METHODS

Reagents and Equipment

Concentrated sulfuric acid: Mallinckrodt, analytical grade. Ethanol, absolute: Commercial Solvents Corporation. Oertel reagent (4) : 2 vol concentrated sulfuric acid was added to 1 vol of 95% absolute ethanol with cooling.

Cuvets: Pyrocell Corporation, 1.5-ml capacity, lo-mm light path.

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