## Abstract TsAF8 is a temperature‐sensitive (TS) mutant of BHK21 cells that arrests at nonpermissive temperatures in the mid‐G~1~ phase of the cell cycle. TsAma^R^‐1 is a TS for growth mutant of CHO cells with a Ts‐ and α‐amanitin‐resistant (Ama^R^) RNA polymerase II activity. Hybrid TsAma^R^‐1 x
Abortive replication of adenovirus 2 in the temperature-sensitive hamster cell mutant tsAF8
✍ Scribed by Takeharu Nishimoto; Case K. Okubo; Heschel J. Raskas
- Book ID
- 119009390
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 939 KB
- Volume
- 78
- Category
- Article
- ISSN
- 0042-6822
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## Abstract A temperature‐sensitive (ts) cell cycle mutant of Chinese hamster fibroblasts with a block in G~1~ was investigated. Attention was on the expression of the activity of three enzymes: ornithine decarboxylase (ODC), S‐adenosylmethionine decarboxylase (SAMDC), and thymidine kinase (TK). OD
## Abstract A method involving short pulses of 5‐bromodeoxyuridine (brUdRib) followed by irraidation with 313 nm light was used to locate the time of replication of certain genes during the cell cycle of two cell lines, AF8 and AL106. AF8, a temperature‐sensitive mutant of BHK21/13 cells, grows at
## Abstract tsAF8 cells are a temperature‐sensitive mutant of BHK cells that arrest at the nonpermissive temperature in the G~1~ phase of the cell cycle. The activity of solubilized RNA polymerase II and its ability to bind [^3^H]‐γ‐amanitin decrease in tsAF8 cells at 40.6°, with a half‐life of ∼ 1