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A useful reagent for derivatization of amino acids

✍ Scribed by Stig Allenmark

Book ID
104222099
Publisher
Elsevier Science
Year
1990
Tongue
French
Weight
246 KB
Volume
31
Category
Article
ISSN
0040-4039

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✦ Synopsis

SUMMARYz N-(Chlorof~yl)ce,

which is readily synthesized by phosgenation of carbazole. has been shown to be an excellent reagent for fluorogenic labelling of amino acids. The derivatization reaction is carried oat at room tempemtore in a borate buffer of pH 9 and yields highly fluorescent derivatives with gocd chromatographic prop&es. Further, the derivative of amino acid enaotiomezs are well separated on BSA-silica (Resolvosil@) analytical columns.

Due to its well-known photophysical properties (strong fluorescence and long phosphotescence),t carbazole (1) is an interesting starting material for the synthesis of derivatization reagents. Since it is a

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## Abstract Phanquinone (chemically: 4,7‐phenanthroline‐5,6‐dione) was applied as an original precolumn derivatization reagent for amino acids followed by separation using MEKC with UV detection (240 nm). The derivatization reaction was carried out at 68Β°C in the presence of aqueous phosphate buffe