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A fully automated amino acid analyzer using NBD-F as a fluorescent derivatization reagent

✍ Scribed by Chiaki Aoyama; Tomofumi Santa; Makoto Tsunoda; Takeshi Fukushima; Chieko Kitada; Kazuhiro Imai


Book ID
101758487
Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
177 KB
Volume
18
Category
Article
ISSN
0269-3879

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Amino acid analysis using 1-naphthylisoc
✍ Amos Neidle; Miriam Banay-Schwartz; Shirley Sacks; David S. Dunlop 📂 Article 📅 1989 🏛 Elsevier Science 🌐 English ⚖ 550 KB

A method which uses 1-naphthylisocyanate as an HPLC precolumn derivatization reagent for amino acid analysis is described. Derivatization is carried out by adding the isocyanate dissolved in dry acetone to a buffered amino acid solution followed by extraction of the excess reagent with cyclohexane.