The reverse transcription and polymerase chain reaction technique (RT-PCR) was assessed for the quantification of changes in mRNA levels from primary astrocyte cultures. The effects of dibutyryl cyclic AMP (dBcAMP) on glial fibrillary acidic protein (GFAP) mRNA and the effects of tumor necrosis fact
A Technique for Isolating Single Cells for Analysis by Reverse Transcription Polymerase Chain Reaction
โ Scribed by M.C. Harbeck; P.L. Rothenberg
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 386 KB
- Volume
- 230
- Category
- Article
- ISSN
- 0003-2697
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## Abstract There are several methods available to analyze the mRNA concentration quantitatively. Among them, the competitive reverse transcription (RTโ)PCR method is very useful. For this method, Cy5โlabeled primers were used, and after gel electrophoresis in 7โ M urea, the Cy5โlabeled singleโstran