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One-Tube Protocol for Single-Cell Reverse Transcriptase-Polymerase Chain Reaction

โœ Scribed by Lizhen Yan; Gregory Kaczorowski; Martin Kohler

Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
164 KB
Volume
304
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

into blocks. The resulting blocks were thick sectioned (approximately 2 mm), mounted on glass microscope slides using Epon 812, and then observed using confocal laser scanning microscopy.

Sheaths of filamentous cyanobacteria, Schizothrix sp., among carbonate sand grains in marine stromatolite sections were clearly labeled by fluorescein. Figures 1a and 1b show the presence of carboxyl and sulfhydryl groups on the sheath of Schizothrix sp. In conclusion, this method could be applicable to labeling polymers such as alginate-containing uronic acids and could serve as a great tool in studying a complex structure of EPSs containing carboxyl and sulfhydryl groups.

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