𝔖 Bobbio Scriptorium
✦   LIBER   ✦

One-Step Duplex Reverse Transcription–Polymerase Chain Reaction for Quantitative Assessment of RNA Degradation

✍ Scribed by Michio Sugita; Jerry L. Haney; Robert M. Gemmill; Wilbur A. Franklin


Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
78 KB
Volume
295
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


A New Quantitative Method of Real Time R
✍ Weihong Liu; David A. Saint 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 108 KB

Real-time reverse transcription (RT) PCR is currently the most sensitive method for the detection of low-abundance mRNAs. Two relative quantitative methods have been adopted: the standard curve method and the comparative C T method. The latter is used when the amplification efficiency of a reference

Analysis of plasma viral RNA levels duri
✍ T. Mirawati Sudiro; Jaroslav Zivny; Hiroaki Ishiko; Sharone Green; David W. Vaug 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 277 KB

There is increasing recognition of the potential importance of viral burden in the pathogenesis of dengue hemorrhagic fever (DHF). There is little data available, however, describing the kinetics of viral replication in humans with natural dengue virus (DV) infection. Standard procedures for measuri

A new method of competitive reverse tran
✍ Rieko Oba; Yasuko Kudo; Naomi Sato; Reiko Noda; Yuzuru Otsuka 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 197 KB

## Abstract There are several methods available to analyze the mRNA concentration quantitatively. Among them, the competitive reverse transcription (RT‐)PCR method is very useful. For this method, Cy5‐labeled primers were used, and after gel electrophoresis in 7 M urea, the Cy5‐labeled single‐stran

Quantitation of Cytosine Deaminase mRNA
✍ C.Ryan Miller; Allen N. Gustin; Donald J. Buchsbaum; Selwyn M. Vickers; Upender 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 451 KB

Cytosine deaminase/5-fluorocytosine (CD/5-FC) is a promising strategy for local cancer gene therapy. We hypothesized that CD expression within tumor cells would be directly related to efficacy and that quantitation of markers of CD expression such as mRNA, protein, and enzyme activity would therefor