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A T7 RNA polymerase-based toolkit for the concerted expression of clustered genes

✍ Scribed by Solmaz Arvani; Annette Markert; Anita Loeschcke; Karl-Erich Jaeger; Thomas Drepper

Book ID
118473800
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
572 KB
Volume
159
Category
Article
ISSN
0168-1656

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Random mntagenesis of the gene for bacte
Random mntagenesis of the gene for bacteriophage T7 RNA polymerase
✍ Rechinsky, Vladimir O. ;Kostyuk, Dmitry A. ;Lyakhov, Dmitry L. ;Chernov, Boris K πŸ“‚ Article πŸ“… 1993 πŸ› Springer 🌐 English βš– 421 KB

Random mutagenesis of the gene for bacteriophage T7 RNA polymerase was used to identify functionally essential amino acid residues of the enzyme. A two-plasmid system was developed that permits the straightforward isolation of T7 RNA polymerase mutants that had lost almost all catalytic activity. It