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A steady-state kinetic study of the ribonuclease A catalyzed hydrolysis of uridine-2′:3′(cyclic)-5′-diphosphate

✍ Scribed by Job Ren-Tze Li; Frederick G. Walz Jr.


Book ID
115702189
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
660 KB
Volume
161
Category
Article
ISSN
0003-9861

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The reactions of a ribonuclease model substrate, the compound uridine-3'-p-nitrophenyl phosphate, have been examined using heavy-atom isotope effects and stereochemical analysis. The cyclization of this compound is subject to catalysis by general base (by imidazole buffer), specific base (by carbona