The yeast one-hybrid system (1H-system) is applied to studies of transcription-linked DNA-protein interactions under in vivo conditions detected by reporter gene expression. By use of a variant of green fluorescent protein (GFP) as a reporter, the new 1H-system generation represents a time-and work-
β¦ LIBER β¦
A Split Enhanced Green Fluorescent Protein-Based Reporter in Yeast Two-Hybrid System
β Scribed by Kyoungsook Park; So Yeon Yi; Chang-Soo Lee; Kyoon Eon Kim; Hyun-Sook Pai; Dai-Wu Seol; Bong Hyun Chung; Moonil Kim
- Publisher
- Springer
- Year
- 2007
- Tongue
- English
- Weight
- 458 KB
- Volume
- 26
- Category
- Article
- ISSN
- 1573-4943
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## Abstract The yeast twoβhybrid (Y2H) assay is a widely used method to study proteinβprotein interactions, a major objective in postgenome research. Despite the tremendous utility of the Y2H assay, several issues including accuracy, speed, automation, and costβeffectiveness limit its application i
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