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Expression and use of the green fluorescent protein as a reporter system in Legionella pneumophila

✍ Scribed by Köhler, R. ;Bubert, A. ;Goebel, W. ;Steinert, M. ;Hacker, J. ;Bubert, B.


Publisher
Springer
Year
2000
Tongue
English
Weight
454 KB
Volume
262
Category
Article
ISSN
0026-8925

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We have constructed three plasmid vectors for the expression of green fluorescent protein (GFP) fusion proteins using the following motif: (His) 6 -GFP-EK-X, where X represents chloramphenicol acetyl-transferase (CAT), human interleukin-2 (hIL-2), and organophosphorous hydrolase (OPH), respectively,