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A spectrophotometric method for the assay of pyrimidine 5′-nucleotidase in human erythrocytes

✍ Scribed by Adolfo Amici; Paolo Natalini; Silverio Ruggieri; Alberto Vita; Giulio Magni


Book ID
114711940
Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
281 KB
Volume
73
Category
Article
ISSN
0007-1048

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📜 SIMILAR VOLUMES


Electrophoretic and kinetic studies of h
✍ Raymonde Rosa; Henri Rochant; Bernard Dreyfus; Colette Valentin; Jean Rosa 📂 Article 📅 1977 🏛 Springer 🌐 English ⚖ 418 KB

A new case of a defect in red cell pyrimidine 5'-nucleotide (P5N) activity was found in a large family from Guadeloupe in the West Indies. The propositus presented a characteristic hemolytic anemia with red cell basophilic stippling, an increased GSH level, and a shift of the peak in absorbance of n

Kinetic and electrophoretic studies of h
✍ Kenji Shinohara; Kouichi R. Tanaka 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 292 KB

The mutant enzyme of a patient with hereditary pyrimidine 5'-nucleotidase deficiency was analyzed biochemically. Partially purified by DEAE-Sephadex and concentrated by ultrafiltration, the enzyme had a high Km for the substrate uridine monophosphate. Utilization of the substrate cytidine monophosph