A spectrophotometric method for the assay of pyrimidine 5′-nucleotidase in human erythrocytes
✍ Scribed by Adolfo Amici; Paolo Natalini; Silverio Ruggieri; Alberto Vita; Giulio Magni
- Book ID
- 114711940
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 281 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0007-1048
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📜 SIMILAR VOLUMES
A new case of a defect in red cell pyrimidine 5'-nucleotide (P5N) activity was found in a large family from Guadeloupe in the West Indies. The propositus presented a characteristic hemolytic anemia with red cell basophilic stippling, an increased GSH level, and a shift of the peak in absorbance of n
The mutant enzyme of a patient with hereditary pyrimidine 5'-nucleotidase deficiency was analyzed biochemically. Partially purified by DEAE-Sephadex and concentrated by ultrafiltration, the enzyme had a high Km for the substrate uridine monophosphate. Utilization of the substrate cytidine monophosph