Analysis of human erythrocyte 5‘-nucleotidases in healthy individuals and a patient deficient in pyrimidine 5’-nucleotidase
✍ Scribed by D. M. Swallow; I. Aziz; D. A. Hopkinson; S. Miwa
- Book ID
- 115249062
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 362 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0003-4800
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📜 SIMILAR VOLUMES
A new case of a defect in red cell pyrimidine 5'-nucleotide (P5N) activity was found in a large family from Guadeloupe in the West Indies. The propositus presented a characteristic hemolytic anemia with red cell basophilic stippling, an increased GSH level, and a shift of the peak in absorbance of n
The mutant enzyme of a patient with hereditary pyrimidine 5'-nucleotidase deficiency was analyzed biochemically. Partially purified by DEAE-Sephadex and concentrated by ultrafiltration, the enzyme had a high Km for the substrate uridine monophosphate. Utilization of the substrate cytidine monophosph