A sensitive fluorometric assay for avidi
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Henry J. Lin; Jack F. Kirsch
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Article
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1977
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Elsevier Science
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English
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The quenching of the tryptophan fluorescence of avidin by biotin forms the basis for a rapid, sensitive assay for biotin and avidin in solutions free of fluorescent contaminants. Avidin solutions are assayed by titration with a standard biotin solution until no further decrease in fluorescence at 35