𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A spectrophotometric assay for biotinbinding sites of immobilized avidin

✍ Scribed by Violeta G. Janolino; Javier Fontecha; Harold E. Swaisgood

Book ID
112893822
Publisher
Springer-Verlag
Year
1996
Tongue
English
Weight
391 KB
Volume
56
Category
Article
ISSN
0273-2289

No coin nor oath required. For personal study only.

πŸ“œ SIMILAR VOLUMES

A spectrophotometric assay for soluble a
A spectrophotometric assay for soluble and immobilized N-hydroxysuccinimide esters
✍ Talia Miron; Meir Wilchek πŸ“‚ Article πŸ“… 1982 πŸ› Elsevier Science 🌐 English βš– 190 KB

A quantitative spectrophotometric assay for N-hydroxysuccinimide and its esters based on the formation of a chromophore upon titration with mild base has been developed. The maximum absorbance for the chromophore is at 260 nm with an extinction coefficient of 9700 M-' cm-'. The method can be used fo

Interaction of Immobilized Avidin with a
Interaction of Immobilized Avidin with an Aequorin–Biotin Conjugate: An Aequorin-Linked Assay for Biotin
✍ Agatha Feltus; Sridhar Ramanathan; Sylvia Daunert πŸ“‚ Article πŸ“… 1997 πŸ› Elsevier Science 🌐 English βš– 215 KB

to labeled biotin results in an inhibition of the signal Biotinylated recombinant aequorin was used in the generated by the label (3-8). The higher the amount development of a heterogeneous bioluminescence of free biotin present, the less labeled biotin is bound binding assay for biotin. This assay