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A spectrophotometric assay for soluble and immobilized N-hydroxysuccinimide esters

✍ Scribed by Talia Miron; Meir Wilchek

Book ID
102628392
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
190 KB
Volume
126
Category
Article
ISSN
0003-2697

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✦ Synopsis

A quantitative spectrophotometric assay for N-hydroxysuccinimide and its esters based on the formation of a chromophore upon titration with mild base has been developed. The maximum absorbance for the chromophore is at 260 nm with an extinction coefficient of 9700 M-' cm-'. The method can be used for measuring the active esters on carriers and their interaction with amines during peptide synthesis.

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Simple and sensitive spectrophotometric and radiochemical procedures are described for the assay of acetyl-CoA:arylamine N-acetyltransferase (NAT; EC 2.3.1.5), which catalyzes the reaction acetyl-CoA + arylamine----N-acetylated arylamine + CoASH. The methods are applicable to crude tissue homogenate