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A Specific Picomolar Hybridization-Based ELISA Assay for the Determination of Phosphorothioate Oligonucleotides in Plasma and Cellular Matrices

✍ Scribed by Xiaohui Wei; Guowei Dai; Guido Marcucci; Zhongfa Liu; Dale Hoyt; William Blum; Kenneth K. Chan


Book ID
106492776
Publisher
Springer US
Year
2006
Tongue
English
Weight
976 KB
Volume
23
Category
Article
ISSN
0724-8741

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An ultrasensitive noncompetitive hybridization-ligation heterogeneous enzyme-linked immunosorbent assay was developed for the quantitation of antisense phosphorothioate oligodeoxynucleotides in plasma using a 96-well plate format. The principle of the assay is based on heterogeneous noncompetitive b